Cen6/arsh4

Author: mfox

August undefined, 2024

WebThe plasmid has been used to construct the plasmids pSA50, pSA52, pSA53, and pSA54. It contains the Candida albicans LYS5MX4 gene as marker for deletion of yeast genes and … http://genome-www.stanford.edu/vectordb/vector_descrip/COMPLETE/PRS314.SEQ.html

pRS314 plasmid in E. coli - 77143 ATCC

WebMembers of the Fusarium solani species complex are filamentous fungi that can act as pathogens to many crops and animals. Although relevant, a robust molecular toolbox is missing for the ... WebIn the BY4741 haploid strain, the low-copy, CEN6/ARSH4 plasmids ranged from 2 to 5 copies per cell and the high copy 2μ plasmids with auxotrophic markers had a wide … ronald burgundy

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WebAddition of yeast CEN6-ARSH4 and partial HIS3, Phaeodactylum tricornutum ShBle cassette, origin of transfer from RP4 Vector type Synthetic Biology ; : Episomal vector for Phaeodactylum tricornutum Selectable markers Zeocin, HIS3 Growth in Bacteria Bacterial Resistance (s) Ampicillin and Tetracycline, 100 & 10 μg/mL Growth Temperature 37°C WebMar 7, 2024 · In the yeast segregation system described in this chapter, the expression plasmids p416MET25 and p425PGK were used (Fig. 4A and B) (26,27). p416MET25 contains the CEN6/ARSH4 sequence, the URA3 marker for selection of plasmid-bearing cells in medium lacking uracil, a MET25 promoter that can be turned off with 2 mM … WebThe CEN6/ARSH4 sequence is a 518 bp hybrid DNA fragment that contains a yeast centromere sequence (CEN) and an autonomously replicating sequence (ARS) (Sikorski … ronald burkle foundation

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WebThis website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies. WebCasein kinase II (CKII) 1 1The abbreviations used are: CKII, casein kinase II; DAPI, 4′,6′-diamidino-2-phenylindole; 5-FOA, 5-fluoroorotic acid. is an essential, highly conserved, acidic-directed protein kinase that is ubiquitous in eukaryotic organisms ( 1 , 2 , 3 , 4 ). ronald burke blue point nyWebCC CEN6 - ARSH4. CC A genomic HincII/PstI fragment (1.002 kb) containing the TRP1 gene was CC inserted into the NdeI site and a cassette containing CEN6 and the ARS CC associated with histone 4 (ARSH4) was inserted into the AatII site of All ends were blunted. ronald burnett obituary

"WebThe pYC2/NT vectors contain the CEN6/ARSH4 sequence (Sikorski and Hieter, 1989) for maintenance and replication in yeast. The CEN6/ARSH4 sequence is a 518 bp hybrid DNA fragment that contains a yeast centromere sequence (CEN) and an autonomously replicating sequence (ARS) (Sikorski and Hieter, 1989). " - Cen6/arsh4

Cen6/arsh4

Construction of the AMT gene deletion vector pKO-geneY. (a) …

http://www.marcottelab.org/users/BCH394P_364C_2024/OneStepAssemblyInYeast.pdf WebJan 2, 2007 · There are two common centromere sequences, CEN4 and CEN6. CEN4 is found in most yeast centromere-containiing vectors, such as pYAC4. These vectors … OpenWetWare is an effort to promote the sharing of information, know-how, and … Current Protocols Most comprehensive source of protocols ranging from … Moving toward a better OWW Materials Page. Please visit the Materials Talk … This page was last edited on 9 March 2024, at 01:12. Content is available under … Pages in category "Yeast" The following 42 pages are in this category, out of 42 total.

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WebThe vector additionally contained ampicillin resistance marker Amp (R) for selection in E. coli, tryptophan selection marker TRP1 for selection in S. cerevisiae, pUC origin of replication for E.... WebCEN6/ARSH4, →, 5012-5526 Terminator CYC1, ->, 2005-2265 Handling information. Host. Escherichia coli HB101 . Medium. ATCC Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin. Temperature. 37°C. Handling notes. Restriction digests of the clone give the following sizes (kb ...

WebThe CEN6/ARSH4 sequence is a 518 bp hybrid DNA fragment that contains a yeast centromere sequence (CEN) and an autonomously replicating sequence (ARS) (Sikorski and Hieter, 1989). The CEN6 sequence is derived from the CEN6 locus of yeast chromosome 6 (Panzeri and Philippsen, 1982) while the ARSH4 sequence is derived … Web中国微生物资源共享平台,微生物菌种保藏中心,泰斯拓生物提供菌株,atcc菌株,菌株,质粒载体,慢病毒，细菌，真菌,支原体,衣原体,dsmz,ccug,atcc,分子技术试验,相关的技术服务,咨询电话0574-87917803

WebCamptothecin is a potent anti-tumor drug that specifically targets DNA topoisomerase I by reversibly stabilizing a covalent enzyme-DNA intermediate (reviewed in Refs. 2 , 4 , 5 , 6 , 7 ). In the yeast Saccharomyces cerevisiae, DNA topoisomerase I has been shown to constitute the cellular target of the drug ( 8 , 9 , 10 , 11 , 12 , 13 , 14 ). WebOne of a series of pBluescript-based centromere vectors (ATCC 77142-77145, 77157-77158) differing in the yeast selectable marker gene. YC-type centromere vector …

WebFeb 9, 2015 · Small and uncharged glycerol is an important molecule for yeast metabolism and osmoadaptation. Using a series of S. cerevisiae BY4741-derived mutants lacking genes encoding a glycerol exporter (Fps1p) and/or importer (Stl1p) and/or the last kinase of the HOG pathway (Hog1p), we studied their phenotypes and various physiological …

Webfor maintenance and replication in yeast. The CEN6/ARSH4 sequence is a 518 bp hybrid DNA fragment that contains a yeast centromere sequence (CEN) and an autonomously … ronald burns obituaryWebMay 1, 2024 · The plasmid pBBR1-Clover-CEN has been constructed by introducing an additional yeast derived sequence CEN6-ARSH4-HIS3 fragment into pBBR1-Clover-Hyg … ronald busch obituaryWebCEN6/ARSH4plasmidpRS412andthe2ì plas-mid pRS422 were created by ligation of the 1246bp ApaLI fragment of pRS402 and the 3781bp ApaLI fragment of pRS402 with … ronald burson carrollton gaWebDescription. 酵母でタンパク質を過剰発現させる方法. 本発明は、例えば、酵母でタンパク質を過剰発現させることによりタンパク質を生産する方法に関する。. これまでに酵母 … ronald burson obituaryWebSecreted protease activity was characterized from 44 ocular clinical isolates, and a higher frequency of protease-positive The Gram-negative bacterium and opportunistic pathogenSerratia marcescenscauses ocular infections in healthy individuals. ronald burnsWebPlasmids pPtPuc1-4 differ in whether they have the P. tricornutum-derived region from p0521s (Pt R1R2, green) or the yeast-derived CEN6-ARSH4-HIS3 (yellow). Other plasmid features present in all... ronald burrows jr obituary ronald burns pitmaster