WebDec 6, 2024 · We use high-resolution Micro-C, ChIP-seq and nascent and total transcript profiling to show that Enhancer-Promoter (E-P) interactions are largely insensitive to acute depletion of CTCF, cohesin, WAPL and YY1. We performed ChIP-seq, RNA-seq, mNET-seq, and Micro-C in mESCs with either CTCF (clone C58), the cohesin subunit RAD21 … WebAug 17, 2024 · The subunit RAD21 was selected as representative of Cohesin. The ZNF143-binding sites were classified into three classes, isolated ZNF143, ZNF143-CTCF, and ZNF143-CTCF-RAD21, to represent ZNF143-binding sites only, ZNF143 and CTCF co-binding sites without RAD21, and ZNF143, CTCF, and RAD21 co-binding sites …
A cohesin cancer mutation reveals a role for the hinge domain in …
WebAug 31, 2016 · Triple sites, CTCF-RAD21 double sites, and CTCF singleton sites were divided into three phylogenetic categories: Mouse only; shared in mouse and rat … WebReduced RAD21 destabilizes high-level gene amplification by disrupting pre-replication complex bindings in human cancers with chromosomal instability. found well-positioned CTCF and RAD21 peaks approximately 60-80 bp upstream of the TSS in the unidirectional genes. The peak heights were related to the amount of antisense transcription. bithc affianco
Entry - *300826 - STROMAL ANTIGEN 2; STAG2 - OMIM
Web5.1 CTCF genome organizer and the cohesin complex. CTCF (CCCTC-binding factor) is a multiple zinc finger protein that exerts diversified functions under different genomic contexts. CTCF was first isolated and cloned on the basis of its ability to bind to highly divergent 50–60 bp sequences within the promoter region of the chicken Myc gene ... Web1 day ago · The same study observed increased distances between anchors into another 500 kb loop upon RAD21 acute depletion (Figures 2 A and 2D). In the other two works, live-cell imaging and modeling approaches were combined to characterize the dynamics of loop formation and stability and to dissect the relative contribution of cohesin, CTCF, and … WebThe Student's t-test p-value for CTCF, RAD21, and SMC3 ChIP-seq peak counts between the two types of boundaries was 9.20 × 10 −71 , 1.94 × 10 −22 , and 1.20 × 10 −41 , respectively. (d-f ... data analyst background