Flp-in-293
WebThe Flp-In™ T‑REx™-293 cell line contains two stably, independently integrated plasmids which exhibit the following features: • The pFRT/lacZeo plasmid introduces a single FRT … WebCell information for Flp-In-293. Find diseases associated with this biological target and compounds tested against it in bioassay experiments. This application requires Javascript.
Flp-in-293
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WebDec 5, 2013 · By using the conditional promoter in the Flp-In-Trex vector, functional expression of Npt2A was achieved by doxycycline induction in HEK293 cells. The EGFP tagged and non-tagged, inducible stable hNpt2A-HEK293 cell lines afforded development of a robust phosphate uptake assay mediated by hNpt2A, which can be used to screen … WebFlp-In ™ 293 T-REx 细胞系设计用于快速生成稳定细胞系，确保通过 Flp-In™ 表达载体对您的目标蛋白质进行均一表达。这些细胞在转录活性基因组基因座处包含一个稳定整合的 …
WebFlp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that ensure homogenous expression of your protein of interest from a Flp-In™ expression vector. … Webpage 13). The Flp-In™ T-REx™ Core Kit manual is also available for downloading at www.lifetechnologies.com. You may use the Flp-In ™ T-REx -293 cell line as a host to …
Webjor components of the Flp-In™ T-REX™ System include: • A Flp-In™ target site vector, pFRT/lacZeo, for generation of a Flp-In™ host cell line containing an integrated FRT site. … WebMar 24, 2024 · It utilizes human Flp-In TM cells, which genomically express a Protein A-tagged version of the human peroxisomal import receptor PEX5L. Native soluble and membrane-bound complexes containing PEX5L can thereby be isolated via a well-known affinity-based strategy. ... Flp-In™ 293 (Invitrogen), stably expressing the target protein …
WebI am trying to generate stable cell line by using Flp-In 293 T-REx cells. I use 9:1 (w/w) ratio of pOG44:pcDNA-GOI and lipofectamine as a transfection reagent.
WebMethods: The human P-gp (ABCB1/Flp-In™-293) stable expression cells were established by using the Flp-In™ system. The effects of tenulin and isotenulin on cell viability were evaluated by SRB assays in established cell lines, sensitive cancer cell line (HeLaS3), and resistant cancer cell line (KB-vin). ... philhealth process flowWebArticle Snippet: The plasmid containing this locus was then transfected into HEK-293 TRex Flp-In cells (ThermoFisher) along with a pOG44 plasmid encoding a Flp-recombinase using Turbofectin according to manufacturer’s protocols. Techniques: Activation Assay, Fluorescence, Transfection. philhealth premium rateWebJun 13, 2016 · The cytotoxicity test demonstrated that IC50 value of atorvastatin to inhibit the growth and/or viability of Flp-In-293/ABCB1 (2677G) cells was 5.44 ± 0.10 mM, which was significantly lower than those in Flp-In-293/ABCB1 (2677 T) (6.02 ± 0.07 mM) and Flp-In-293/ABCB1 (2677A) cells (5.95 ± 0.08 mM). ... philhealth process hospitalWebFlp-In 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In expression vector. Targeted … philhealth processing timeWebFlp-In™ 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In™ expression vector. These cells … Flp-In™ 293 cell line is designed for rapid generation of stable cell lines that … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes philhealth profileWebHi, we have done the opposite, conditioned HEK293 (Flp-In) cells to grow in suspension, so I think yes. You can try to reverse the conditioning process detailed here, see page 7: Article Affinity ... philhealth processingWebMay 18, 2016 · Hek 293 cell line, Doxycycline, expression, tissue culture, cell culture, cancer, cell cycle checkpoint. philhealth printable form