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Glutathione Sepharose 4B

WebProperties of Glutathione Sepharose High Performance resin. The glutathione ligand is coupled via a 10-carbon linker to highly cross-linked 6% agarose, 34 µm beads. ... Glutathione Sepharose High Performance (HP) is also available in 1 mL and 5 mL … WebGE Healthcare glutathione sepharose 4b beads Glutathione Sepharose 4b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ... GE Healthcare hiload 1610 phenyl sepharose hp hydrophobic interaction chromatography column Hiload 1610 Phenyl Sepharose Hp Hydrophobic … bambam backup dancer

Purification Using GSTrap™ HP, GSTrap™ FF, and …

WebFeb 2, 2007 · Glutathione Sepharose 4B comes as a 60% slurry in 20% ethanol; a 50% slurry should be prepared before use. It is to be stored at 4-8ºC. The other reagents required for purification are routinely-used chemicals. To prepare the 50% slurry, 1.3 ml of the 60% slurry is placed in a 15 ml tube and 10 ml of chilled PBS is added into it, mixed gently ... WebGlutathione Sepharose ® High Performance is easy to use for one-step purification of GST-tagged proteins. The following data show the results from experiments using both … WebGlutathione Sepharose Bed Volume* 10 ml 2 ml 200 µl 10 µl 1X PBS** 100 ml 20 ml 2 ml 100 µl Glutathione Elution Buffer 10 ml 2 ml 200 µl 10 µl *To obtain the desired bed volume, use twice the volume of 50% Glutathione Sepharose slurry prepared in Procedure A (i.e. 1 ml of 50% Glutathione Sepharose slurry will give a bed volume of 0.5 ml). bamba mbacke dieng