WebLow pressure liquid chromatography (LPLC) is a chromatographic technique that operates at low pressures to drive the mobile phase onto the column containing a stationary phase by the action of a pump. WebThe original HPLC method was performed on an LC with 1.0 mL system volume. Converting the system volume to column volumes will determine the length of isocratic hold necessary on the UPLC method to replicate the same peak profile. HPLC system volume = 1.0 mL = 0.40 cv Scaling a 10 μLinjection on 4.6x150 mm to 2.1x50 mm 0.17 2.49 10 μL x

UHPLC/HPLC Method Translator: Phenomenez

WebExperience seamless method transfer by using ISET to predict what chromatograms receiving labs will observe, independent of what provider of LCs they use Accelerate your instrument-to-instrument method transfer. Simply emulate the LC system on which the original method was developed and achieve the same retention times and peak resolution WebHPLC Method Transfer Calculator Calculate the saving in run time and solvent consumption when transferring a method from HPLC conditions to UHPLC conditions. Obtain guidance on change in back pressure as well as how to adapt injection volume and gradient conditions. garthorpe scunthorpe

Preparative HPLC Column Method Transfer Tool - US

Web13 jul. 2024 · Gradient program used in HPLC method was converted into short run time RRLC using HPLC method transfer calculator (Thermo Fisher Scientific, Waltham, MA, USA) [ 14 ]. The new gradient program was; time (min)/mobile phase B (%); 0.01/0, 5/20, 6/30, 7/35, 8/35, 9/0 and 10/0 with a flow rate of 2.0 mL min −1. Injection volume was 5 µL. Web10 nov. 2024 · Legacy HPLC method needs to be translated to a new column or LC system (HPLC to UHPLC or Analytical to Preparative LC). Requirements Install the most recent … WebPopular answers (1) There are many free scaling calculators on the web, but be careful using them as they all make "assumptions" which may or may not apply to your specific HPLC system, column or ... garth o\\u0027brien